CHARDAKOV METHOD PDF

Understanding the osmotic potential of plant cells is a key part of understanding cellular processes. Here we present two methods of determining osmotic potential of plant tissues using potatoes. Method one, the standard protocol for measuring weight change of tissues in varying osmotic solutions, is reliable but does not demonstrate the changing solute potentials. Sign up now. Investigating osmosis: measuring the water potential of a potato cell. Tags: Post 16 , Plant cells and tissues.

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Abstract: The results and characteristics of water potential measurements with four different methods, namely the Chardakov method, Pressure chamber, in situ hygrometry and leaf disc hygrometry were compared on several plants species.

Experimental results showed that the former 3 methods produced similar results, but the leaf disc hygrometry yielded significant different results from the other 3 methods. Further investigation showed that the results with the leaf disc hygrometry varied considerably with the equilibrium time and was not able to show the actual water potentials of the plants.

The Chardakov method was time-consuming, and its efficiency was very low. Pressure chamber was able to provide accurate and reliable measurements of plant water potentials if the sample water loss could be avoided and the repetitious samples were available. While the in situ hygrometry showed the merits such as more reliable results, non-destructive to plants, no harsh prerequisites for plants and could measure the same spot of a plant continuously.

Key words: water potential, pressure chamber Scholander bomb , in situ hygrometry, leaf disc hygrometry, chardakov method. Scientia Silvae Sinicae, , 48 12 : PDF PC Knowledge 0. Related Articles Scientia Silvae Sinicae, , 54 12 : Scientia Silvae Sinicae, , 53 9 : Scientia Silvae Sinicae, , 53 5 : Scientia Silvae Sinicae, , 52 6 : Scientia Silvae Sinicae, , 48 1 : Scientia Silvae Sinicae, , 47 3 : Scientia Silvae Sinicae, , 12 9 : Scientia Silvae Sinicae, , 40 5 : Scientia Silvae Sinicae, , 36 4 : Recommended Articles.

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Chardakov method

Chardakov Method for Determining Water Potential. This method depends on the change in density in a solution that occurs after a tissue has been immersed in it. The solution gains or looses water depending on the water potential of the tissue. If the density of a solution does not change no net movement of water then this solution has the same water potential as the tissues that were incubated in it.

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Home Page Research Chardakov method. Chardakov method Words 6 Pages. Question: investigate the water potential of potato tissue? Introduction All cells require essential materials to ensure their survival.

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